Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7233798 | Biosensors and Bioelectronics | 2014 | 6 Pages |
Abstract
A sensitive and facile electrochemical biosensor has been developed for monitoring the protein nitration damage affected by the nitro free radicals(NO2). The NO2 radicals is generated from hemin-catalyzed oxidation of nitrite (NOâ2) in the presence of hydrogen peroxide (H2O2). In this work, nanocomposite films of graphene-gold nanoparticles (EG-AuNPs) were modified on the glassy carbon electrode by electropolymerization. Bovine serum albumin (BSA) was then further assembled on EG-AuNPs film through Au-S bond. The damage of BSA molecule was caused by the NO2 radicals which was generated from the NaNO2/hemin/H2O2 nitration reagent. The differential pulse voltammetry was used to detect the damage of BSA molecule. Fluorescence spectra and circular dichroism spectrum further confirmed the nitration damage of BSA. Moreover, the lowest concentration at which the BSA damage was detected is 28.9 µM NOâ2 or H2O2, and the volume ratio of NOâ2 and H2O2 was 1:1 in the hemin/NOâ2/H2O2 nitration reagent. The results demonstrated that the proposed electrochemical method can be used to detect protein damage affected by nitration reagent. The developed electrochemical biosensor is envisioned to have promising applications in protein damage studies.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Yanjuan Tang, Yanjie Guo, Lu Zhang, Jiye Cai, Peihui Yang,