Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7233821 | Biosensors and Bioelectronics | 2013 | 7 Pages |
Abstract
This paper demonstrates a proof-of-principle for a new signal transduction method for protein detection called Bead Assembly Magnetorotation (BAM). BAM is based on using the target protein to mediate the formation of aptamer-coated 1 μm magnetic beads into a bead assembly, formed at the bottom of a 1 μL hanging droplet. The size, shape and fractal dimension of this bead assembly all depend on the protein concentration. The protein concentration can be measured in two ways: by magnetorotation, in which the rotational period of the assembly correlates with the protein concentration, or by fractal analysis. Additionally, a microscope-free magnetorotation detection method is introduced, based on a simple laser apparatus built from standard laboratory components. In this paper, we chose to focus on the protein thrombin, a popular choice for proof-of-principle work in this field.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Ariel Hecht, Patrick Commiskey, Nicholas Shah, Raoul Kopelman,