| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7233949 | Biosensors and Bioelectronics | 2013 | 6 Pages |
Abstract
In this research, we report the development of an aptamer based SPR biosensor for the detection of catalase in milk samples with minimal sample preparation. A biotin tagged aptamer was immobilized onto a gold surface by affinity capture. A limit of detection (LOD) in the nanomolar range (20.5Â nM, RSD: 15.2%) was found and a dynamic range of 15-1000Â nM was established for catalase in buffer and the aptamer showed good specificity toward catalase. This biosensor has the potential to be used in the detection of catalase in milk samples, a key indicator of mastitis disease in milk.
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Authors
Jon Ashley, Sam F.Y. Li,