Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7234033 | Biosensors and Bioelectronics | 2013 | 6 Pages |
Abstract
A highly sensitive label-free immunosensor for the detection of kanamycin had been developed using silver hybridized mesoporous ferroferric oxide nanoparticles (Ag@Fe3O4 NPs) and thionine mixed graphene sheet (TH-GS). TH was used as an electron transfer mediator. The electrical signal was greatly improved in the presence of GS due to its good electron-transfer ability. With the advantages of large specific surface area and excellent electrical conductivity, Ag@Fe3O4 NPs could immobilize more antibodies of kanamycin and promote the electron transfer. Cyclic voltammetry and square wave voltammetry were used to characterize the recognition of kanamycin. The proposed immunosensor showed good performances such as low detection limit (15 pg mLâ1), wide linear range (from 0.050 to 16 ng mLâ1), short analysis time (3 min), high stability, and good selectivity in the detection of kanamycin. The immunosensor was evaluated for pork meat sample, receiving satisfactory results.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Shujun Yu, Qin Wei, Bin Du, Dan Wu, He Li, Liangguo Yan, Hongmin Ma, Yong Zhang,