Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7234357 | Biosensors and Bioelectronics | 2013 | 6 Pages |
Abstract
⺠The immunospot assay for Dengue fever overcomes time limitation problems observed for ELISA tests. ⺠The procedure requires only 45-60 min and requires only one antibody for detection. ⺠The method uses 4 μL serum sample and allows multiple sample analysis. ⺠The detection is based on antibody coated fluorescent nanoparticles. ⺠The detection limit for Dengue NS1 protein is 5.2 ng/mL and comparable to ELISA tests.
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Authors
Elisângela M. Linares, Claudio S. Pannuti, Lauro T. Kubota, Stefan Thalhammer,