| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7234621 | Biosensors and Bioelectronics | 2013 | 6 Pages |
Abstract
⺠A BRET2-based maltose biosensor was constructed using genetically encoded components. ⺠MBP was flanked by green fluorescent protein (GFP2) and Renilla luciferase variant (RLuc2). ⺠BRET2 was three times more sensitive than FRET for measuring maltose. ⺠The EC50 was 0.37 μM for maltose and the BRET2 response was linear over three orders of magnitude. ⺠Maltose was accurately measured in beer demonstrating potential use in the fermentation industry.
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Authors
Helen Dacres, Michelle Michie, Alisha Anderson, Stephen C. Trowell,