Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7235018 | Biotechnology Reports | 2017 | 5 Pages |
Abstract
Here, we present a low-cost and reliable approach based on the allele specific loop-mediated isothermal amplification (AS-LAMP) coupled to ssDNA functionalized gold nanoparticle (Au-nanoprobe) colorimetric sequence discrimination. The Au-nanoprobe integration allows for the colorimetric detection of AS-LAMP amplification product that can be easily interpreted in less than 15Â min. We targeted a clinical relevant SNP responsible for lactose intolerance (-13910C/T dbSNP rs#: 4988235) to demonstrate its proof of concept and full potential of this novel approach.
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Authors
Fábio Ferreira Carlos, Bruno Veigas, Ana S. Matias, Gonçalo Doria, Orfeu Flores, Pedro V. Baptista,