NORMALISATION OF DNA ARRAY DATA TO FACILITATE THEIR USE IN BIOPROCESS DEVELOPMENT

The application of DNA array is spreading at a very fast rate, although mainly in medical and systems biology studies. However, the recent FDA Process Analytical Technologies initiative provides a very exciting possibility of using transcriptomics, proteomics and metabolomics to gain deeper bioprocess understanding leading to more rapid process development and tighter control of critical process parameters. This contribution introduces a method of normalisation necessary for DNA data analysis usable in such circumstances – i.e. a limited number of experiments under varying operational conditions. We show that this normalisation technique provides more plausible data preparation for further data analysis.