OUR AND CER ESTIMATION IN HIGH DENSITY MAMMALIAN CELL PERFUSION CULTURES

We present methods for real-time estimation of OUR and CER in high density mammalian cell perfusion cultures. These computations are based on global mass balances and do not require kLa data and reactor perturbations. The applicability of this approach was tested in a long-term CHO cell cultivation where pH, temperature and DO were varied over the course of the cultivation. Real time OUR and CER data enable real-time metabolic flux estimation and allow cell physiological state monitoring and manipulation through advanced bioreactor process control.