PAT – PROCESS ANALYTICAL TECHNOLOGY IN CULTIVATION PROCESSES WITH RECOMBINANT ESCHERICHIA COLI

An extensive monitoring system is build up to fully automate and scan production processes of recombinant Escherichia coli for optimal process performance. It is based on in-line, on-line and at-line measurements for substrates and products. MATLABr is connected via OPC OPC Object linked and embedded (OLE) for process control to the SCADASCADA Supervisory control and data acquisition system MFCS/winr to realise on-line calculations of cell specific and volumetric reaction rates, based on the applied analytical systems. For on-line determination of intracellular target protein recombinant GFP is expressed either as single or fusion protein.