Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
724098 | IFAC Proceedings Volumes | 2007 | 4 Pages |
Abstract
We have developed a new probe that measures the oxygen uptake rate (OUR) of mammalian cells, an important indicator for the metabolic state, inside the reactor (in-situ) and in real-time without the need for sampling or complicated analytics. The probe isolates a known volume of cell culture from the bulk inside the bioreactor, monitors the oxygen consumption over time and releases it again. The sample is mixed during the measurement with a new agitation system to keep the cells in suspension and prevent oxygen concentration gradients. The OUR measurement system also doubles as a standard DO probe for process monitoring when it is not performing OUR measurements. This new probe was successfully tested in BHK perfusion cell cultures.
Keywords
Related Topics
Physical Sciences and Engineering
Engineering
Computational Mechanics
Authors
K. Joeris, L. Behr, M. Burnett, T. Scheper, K. Konstantinov,