Article ID Journal Published Year Pages File Type
735852 Optics and Lasers in Engineering 2010 7 Pages PDF
Abstract

Biochips have advanced tremendously for biomedical applications in recent years. New fluorescence imaging methods have been developed for biochip analysis. In this work, a two-channel, quasi-confocal parallel scan fluorescence imaging method is proposed for detection of biochips. An imaging system was developed with one-dimensional optical scan to produce two-dimensional wide-field images, and the system was designed to enable simple exchange of lasers covering different spectral regions. The mechanism of quasi-confocal imaging provides a sensitivity of 0.46 fluorescence molecules per square micron, while parallel scan makes it a high throughput technique for rapid detection of biochips.

Related Topics
Physical Sciences and Engineering Engineering Electrical and Electronic Engineering
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