A LAPS array with low cross-talk for non-invasive measurement of cellular metabolism

Cellular metabolism is ubiquitous biological mechanism involved in many significant physiological processes, and most of the biological cascade reactions are tightly coupled. Non-invasive measurement of cellular metabolism is important to study the metabolism mechanism and drug effect in a long-term detection. In this study, the two processes of heavy doping and thick oxidation fabricated a new light-addressable potentiometric sensor array, which was applied to detect the cellular metabolism induced pH change. The performance of the LAPS was tested by the basic characteristic experiments and renal cell experiments. The result showed that the stability and pH resolution of LAPS array were greatly improved. With the development of the sensor design and fabrication, the LAPS will be a utility tool in the field of biological metabolism.