Article ID Journal Published Year Pages File Type
7556655 Analytical Biochemistry 2018 33 Pages PDF
Abstract
The application of molybdenum disulfide (MoS2) nanosheets has assumed great significance in the design of next-generation biosensors. The immobilization of biomolecules on MoS2 nanosheets has generally been achieved via hydrophobic interactions or through other complicated surface modifications. In this work, we report a novel strategy for electrochemically assisted amine derivatization of MoS2 nanosheets. This newly proposed approach helped to immobilize the MoS2 nanosheets with antibodies via facile EDC/NHS {N-(3-dimethylaminopropyl)-N-(ethylcarbodiimide/N-hydroxysuccinimide)} cross-linking chemistry. The MoS2 nanosheets were first exfoliated and then electrochemically modified with 2-aminobenzylamine. Through a subsequent bioconjugation of the above amine-derivatized MoS2 nanosheets with anti-prostate-specific antigen (PSA) antibodies, an immunosensing device was realized for the detection of the 'prostate specific antigen'. The application of the proposed immunosensor was characterized with a low detection limit (10−3 ng/mL) over a very wide quantitation range (10−3 to 200 ng/mL).
Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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