Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7556655 | Analytical Biochemistry | 2018 | 33 Pages |
Abstract
The application of molybdenum disulfide (MoS2) nanosheets has assumed great significance in the design of next-generation biosensors. The immobilization of biomolecules on MoS2 nanosheets has generally been achieved via hydrophobic interactions or through other complicated surface modifications. In this work, we report a novel strategy for electrochemically assisted amine derivatization of MoS2 nanosheets. This newly proposed approach helped to immobilize the MoS2 nanosheets with antibodies via facile EDC/NHS {N-(3-dimethylaminopropyl)-N-(ethylcarbodiimide/N-hydroxysuccinimide)} cross-linking chemistry. The MoS2 nanosheets were first exfoliated and then electrochemically modified with 2-aminobenzylamine. Through a subsequent bioconjugation of the above amine-derivatized MoS2 nanosheets with anti-prostate-specific antigen (PSA) antibodies, an immunosensing device was realized for the detection of the 'prostate specific antigen'. The application of the proposed immunosensor was characterized with a low detection limit (10â3â¯ng/mL) over a very wide quantitation range (10â3 to 200â¯ng/mL).
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Manil Kukkar, Satish K. Tuteja, Parveen Kumar, Ki-Hyun Kim, Akhshay Singh Bhadwal, Akash Deep,