Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7556666 | Analytical Biochemistry | 2018 | 4 Pages |
Abstract
Quantitative comparative proteomics require accurate and reproducible assessments of total protein concentration. We report a straightforward, cost-effective adaptation of an established commercial method for total protein quantification (EZQâ¢), utilising non-proprietary materials and colloidal Coomassie Brilliant Blue (cCBB) staining to achieve greater reproducibility, equal sensitivity, and optimal linearity of signal within a practical concentration range for proteins in common solubilisation buffers (i.e. for isoelectric focussing and/or SDS-PAGE). This method provided more accurate and precise determinations of total protein concentration in human serum prepared for two-dimensional gel electrophoresis, indicating it may be better suited as the lead-in to most quantitative proteomic analyses.
Related Topics
Physical Sciences and Engineering
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Analytical Chemistry
Authors
Nour Noaman, Jens R. Coorssen,