Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7556667 | Analytical Biochemistry | 2018 | 23 Pages |
Abstract
Effective separation of short DNA fragments is important for the identification of PCR or LAMP products. We investigated the effect of electric field strength, sample plug width, effective length of the capillary, concentration and molecular weight of polymer on the separation performance of small DNA. Results demonstrated that the sample plug played a non-negligible role in the peak broadening. The migration time of DNA was exponentially decreased with the increase of electric field strength. Increasing effective length of capillary, concentration or molecular weight of HEC may improve the separation performance, but it was at the cost of long migration time.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Shuyi Cheng, Ping Wang, Chunxian Tao, Dawei Zhang, Zhenqing Li, Yoshinori Yamaguchi,