Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7556672 | Analytical Biochemistry | 2018 | 4 Pages |
Abstract
In this paper, we have developed a label-free and rapid fluorescence assay for the detection of exonuclease III (exo III) activity via thioflavin T (ThT) as the G-quadruplex inducer. In this assay, a hairpin probe (HP) with a 5â²-guanine-rich (G-rich) sequence is employed as the substrate for exo III. In the presence of exo III, HP can be digested at 3â²-OH termini releasing 5â²-G-rich sequence. Then, the 5â²-G-rich sequence folds into a G-quadruplex, which can be recognized quickly by the ThT dye resulting in an increase in fluorescence emission. This strategy can detect exo III activity as low as 0.5 U/mL. This assay is simple and of low cost without the requirement of labeling with a fluorophore-quencher pair.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Xiafei Jiang, Haisheng Liu, Farjana Yeasmin Khusbu, Changbei Ma, Anqi Ping, Qiling Zhang, Kefeng Wu, Mingjian Chen,