Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7556686 | Analytical Biochemistry | 2018 | 29 Pages |
Abstract
Glycerol kinase (GK) and glycerol-3- phosphate oxidase (GPO) nanoparticles (NPs) were prepared, characterized and immobilized onto pencil graphite (PG) electrode to fabricate an improved amperometric glycerol biosensor (GKNPs/GPONPs/PGE). GKNPs/GPONPs/PGE worked in optimum conditions of pH 7.0, temperature 30â¯Â°C, at an applied potential of â0.3â¯V. The biosensor exhibited wide linear response in a concentration range of glycerol (0.01-45â¯mM) with detection limit 0.0001â¯Î¼M. The biosensor revealed high sensitivity (7.24â¯Î¼AmMâ1cmâ2), low response time (2.5s) and a good agreement with the standard enzymic colorimetric method with a correlation coefficient (R2â¯=â¯0.99). The evaluation study of biosensor offered a good analytical recovery of 98.73% when glycerol concentration was added to the sera sample. In addition, within and between batches study of working electrode showed coefficients of variation as 0.105% and 0.14%, respectively. The application of biosensor is performed in the serum of apparently healthy subject and patients affected by cardiogenic shock. There was a 20% loss in initial activity of biosensor after its regular use over a time period of 180 days, while being stored at 4â¯Â°C.
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Authors
Vinay Narwal, C.S. Pundir,