Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7556700 | Analytical Biochemistry | 2018 | 46 Pages |
Abstract
Different factors affect the long term stability of monoclonal antibodies, among them denaturation or partial denaturation that is often followed by aggregation. Isothermal calorimetry is capable of quantifying the kinetics of denaturation/aggregation of an antibody by measuring the heat that is released or absorbed by the process over a period of days or weeks, at temperatures below its denaturation temperature, Tm. The denaturation/aggregation kinetics of the anti-HIV monoclonal antibody VRC07-523LS was measured by isothermal calorimetry at different concentrations in four different formulation buffers. The measurements were performed at ten degrees below Tm, as determined by differential scanning calorimetry. The formation of aggregates was also followed by size exclusion chromatography at 5â¯Â°C, 25â¯Â°C and 40â¯Â°C over a period of 8-36 weeks. It was observed that the rates measured by isothermal calorimetry correlate quantitatively with those measured by size exclusion chromatography. Since isothermal calorimetry experiments are performed over a period of ten days, it can become a valuable tool for a fast prediction of the best formulations.
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Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Benjamin R. Clarkson, Rajoshi Chaudhuri, Arne Schön, Jonathan W. Cooper, Lisa Kueltzo, Ernesto Freire,