Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7556718 | Analytical Biochemistry | 2018 | 35 Pages |
Abstract
A new Schiff base grafted graphene oxide-magnetic chitosan was utilized as a novel sorbent for extraction and quantification of lead ion in blood samples via dispersive magnetic solid phase extraction. The prepared nanocomposite sorbent was characterized by SEM, TEM, FT-IR, XRD, VSM and EDX and the quantification analysis was performed by microsampling flame atomic absorption spectrometry. The important parameters on the extraction efficiency were thoroughly optimized by means of experimental design. Under the optimized conditions, an aliquot of 50â¯mL of sample (pH 6.3) was extracted utilizing 60â¯mg of magnetic nanoparticles during 30â¯min. The sorbent was afterward desorbed using 1.0â¯mL of 0.8â¯molâ¯Lâ1 HNO3 under fierce vortex for 6â¯min. A preconcentration factor of 20 and an absolute recovery of 40% were provided by the proposed method. The limits of detection (3S/N) and quantification (10â¯S/N) were 0.06â¯ngâ¯mLâ1 and 2.0â¯ngâ¯mLâ1, respectively. An excellent linearity was achieved within the range of (10-800) ng mLâ1 and the regression coefficient was 0.9903. The intra- and inter-day RSDs% were found to be 1.8% and 7.0%, respectively. Furthermore, the method was applied for analysis of blood samples and good accuracies within the range of 97%-108% were obtained.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Shahram Seidi, Mahshid Majd, Maryam Rezazadeh, Maryam Shanehsaz,