Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7556753 | Analytical Biochemistry | 2018 | 45 Pages |
Abstract
The mitochondrial set of proteins is a dynamic system, crucial for multiple functions of this organelle. Differential expression of genes in various tissues, alternative splicing, post-translational modifications, turnover and spatial dynamics of proteins are the factors that influence mitochondrial proteomes increasing their versatility. A wide range of high-throughput proteomic approaches are extensively used for identification, quantification and functional assessment of human and other mammalian mitochondrial proteins. This article reviews the methods and approaches which can be utilized for achieving one or another specific goal in mitochondrial investigations, and the recent advances in application of proteomics to study the roles of mitochondria in tumorigenesis and cancer progression.
Keywords
TMTmTORIMACMAMMRMICATTOFMPTPACAT1Drp1BN-PAGEMfn1ITRAQMDLCPTMsDDAVDAC1MDH21DEESI2D-DIGE2DEMitochondrial DNAm/zMS/MSROStwo-dimensional gel electrophoresisblue native polyacrylamide gel electrophoresisone-dimensional gel electrophoresisstable isotope labeling with amino acids in cell cultureisobaric tags for relative and absolute quantificationImmunoprecipitationEnzyme-linked immunosorbent assayELISAAffinity purificationpost-translational modificationsTandem mass tagsIsotope-coded affinity tagsmtDNADIATime-of-Flight electron transport chainCancerSILACendoplasmic reticulumCo-IPMass spectrometryTandem mass spectrometryMitochondria-associated membranesSELDISurface-enhanced laser desorption/ionizationmatrix assisted laser desorption/ionizationMALDIMitochondrial permeability transition poresMitochondrial malate dehydrogenasemitofusin 1MitochondriaMass-to-charge ratiomultiple reaction monitoringCo-ImmunoprecipitationETcProteomicsdynamin-related protein 1TCA cycletricarboxylic acid cyclehigh-performance liquid chromatographyimmobilized metal affinity chromatographyData independent acquisitionData Dependent AcquisitionMitochondrial quality controlReactive oxygen specieselectrospray ionization
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Lidia Eremina, Natalya Pashintseva, Leonid Kovalev, Marina Kovaleva, Sergey Shishkin,