| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7556872 | Analytical Biochemistry | 2018 | 6 Pages |
Abstract
Since the intra and inter-laboratory comparison of the outcomes of the hydrolytic procedure is the first step toward its standardization, the normalization of the qPCR data by the UV/qPCR ratio seems to be the simplest and most reliable way to allow this. Finally, the supplying (provided with the commercial qPCR kits) of a DNA sample whose degree of degradation is well documented could be helpful in ISO/IEC 17025 validation procedures and in proficiency testing.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Paolo Fattorini, Giorgio Marrubini, Serena Bonin, Barbara Bertoglio, Pierangela Grignani, Elisa Recchia, Paola Pitacco, Francesca Procopio, Carolina Cantoni, Irena ZupaniÄ PajniÄ, Solange Sorçaburu-Cigliero, Carlo Previderè,