Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7556925 | Analytical Biochemistry | 2018 | 7 Pages |
Abstract
In this work, a label-free electrochemical immunosensor was constructed on the base of poly p-phenylenediamine (PPD) and GR nanocomposite (PPD-GR). Screen-printed electrodes modified with PPD-GR nanocomposite and applied to advance enzyme-free and label free electrochemical immunosensor for detection of protein biomarker neuron-specific enolase (NSE). It was found that the PPD-GR nanocomposite exhibits excellent electrocatalytic activity towards ascorbic acid (AA) oxidation as analytical signal based on EC' mechanism. Due to the excellent electrocatalytic activity of PPD-GR nanocomposite, determination of NSE antigen was based on its obstruction to the electrocatalytic oxidation of AA after binding to the surface of electrode through interaction with the anti-NSE. The proposed immunosensor exhibited a wide linear range of 1.0-1000â¯ngâ¯mLâ1, with a low detection limit of 0.3â¯ngâ¯mLâ1. Furthermore, the proposed immunosensor were successfully used for the determination of NSE antigen in human serum samples.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Jafar Amani, Mehdi Maleki, Alireza Khoshroo, Ali Sobhani-Nasab, Mehdi Rahimi-Nasrabadi,