Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7556969 | Analytical Biochemistry | 2018 | 7 Pages |
Abstract
A model based on gelatin for protease activity studies was designed. The model is also extended to study the efficiency of inhibitors in a separate protective layer covering the layer containing the target substrate. A good correlation between protease concentration and the size of erosion wells formed in a plain gelatin layer was observed. Similarly, increased concentration of inhibitors gave a systematic decrease in well area. Kinetic analyses of the two-layer model in a spectrophotometric plate reader with a fixed concentration of substrate in the bottom layer displayed a strict dependence of both inhibitor concentration and thickness of the top “protective” layer. An apparent, but weaker inhibition effect was also observed without inhibitors due to diffusional and erosion delay of enzyme transport to the substrate-containing layer.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Erika Billinger, Gunnar Johansson,