| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7556975 | Analytical Biochemistry | 2018 | 4 Pages |
Abstract
MALDIâTOF MS has developed rapidly into an essential analytical tool for the life sciences. Cinnamic acid derivatives are generally employed in routine molecular weight determinations of intact proteins using MALDIâTOF MS. However, a protein of interest may precipitate when mixed with matrix solution, perhaps preventing MS detection. We herein provide a simple approach to enable the MS detection of such precipitated protein species by means of a “direct deposition method” -- loading the precipitant directly onto the sample plate. It is thus expected to improve routine MS analysis of intact proteins.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Hiroyuki Oshikane, Masahiko Watabe, Toshio Nakaki,