| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7557046 | Analytical Biochemistry | 2018 | 17 Pages |
Abstract
Adherent cells such as mouse RAW cells or human cancer U2OS cells are beneficial to DNA transfection, with 20%-60% transfection efficiency. However, this DNA transfection is rarely used on suspension cells due to its low transfection efficiency (â¤5%). We recently found a new DNA transfection method to increase the efficiency up to 13.5% in suspension cells without PMA treatment. We also found that DNA transfection of human TNFAIP1 or CXCL1 recombinant plasmid DNA in THP-1â¯cells induces a high level of TNF-α protein. Overall, this new method is simple yet efficient and can be used for the overexpression of DNA in suspension cells.
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Authors
Xiaoren Tang, Bushra Aljahdali, Mansour Alasiri, Abdullah Bamashmous, Feng Cao, Serge Dibart, Erdjan Salih,