Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7557083 | Analytical Biochemistry | 2018 | 24 Pages |
Abstract
Escherichia coli O157: H7 (E. coli O157: H7) has become one of the most dangerous foodborne pathogenic bacteria around the world. Currently, because of the tedious, high-cost and stringent laboratory conditions required, the conventional E. coli O157: H7 detection methods, such as culture-based methods and polymerase chain reaction (PCR), have much limitation. Thus, we developed a novel paper-based enzyme-linked immunosorbent assay (p-ELISA) with shorter operation duration, lower cost, relatively higher sensitivity and wider application. This method required less than 3 h and 5 μL of sample to complete the detection. The limit of detection (LOD) for E. coli O157: H7 reached 1 Ã 104 CFU/mL with high specificity. To be more suitable for on-site testing, the readout could be rapidly obtained without any expensive instruments. In this study, we chose E. coli O157:H7 as the representative, and our method could provide a platform for determination of other pathogenic bacteria.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Bo Pang, Chao Zhao, Li Li, Xiuling Song, Kun Xu, Juan Wang, Yushen Liu, Kaiyue Fu, Hao Bao, Dandan Song, Xiangjun Meng, Xiaofeng Qu, Zhuping Zhang, Juan Li,