| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7557325 | Analytical Biochemistry | 2016 | 4 Pages |
Abstract
A new format of a very rapid, low-cost and high-productive analysis based on the acid precipitation of radiolabeled DNA was developed. By contrast to the conventional processing of a large number of GF/C discs, the method employs one GF/C strip containing samples on individual teeth. The strip assay was validated by comparison with the glass fiber disk technique; the efficiency was demonstrated by screening E. coli superproducers and fractions obtained at the steps of Bst DNA polymerase, Large Fragment purification by the protocol we developed. The principle proposed allows simultaneous assaying many samples for the activity of different polymerases.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Maria Yu Kordyukova, Valeriya N. Antipova, Vadim V. Rogachevsky, Nadezhda V. Zyrina,