Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7557509 | Analytical Biochemistry | 2016 | 11 Pages |
Abstract
Alkylating agents are a significant class of environmental carcinogens as well as commonly used anticancer therapeutics. Traditional alkylating activity assays have utilized the colorimetric reagent 4-(4-nitrobenzyl)pyridine (4NBP). However, 4NBP based assays have a relatively low sensitivity towards harder, more oxophilic alkylating species and are not well suited for the identification of the trapped alkyl moiety due to adduct instability. Herein we describe a method using water as the trapping agent which permits the trapping of simple alkylating electrophiles with a comparatively wide range of softness/hardness and permits the identification of donated simple alkyl moieties.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Philip G. Penketh, Krishnamurthy Shyam, Raymond P. Baumann, Rui Zhu, Kimiko Ishiguro, Alan C. Sartorelli, Elena S. Ratner,