Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7558120 | Analytical Biochemistry | 2015 | 32 Pages |
Abstract
The strongest fluorescent response (up to 70 times) and the same sensitivity to aggregates of both proteins were exhibited by the dye D-51 carrying N-sulfoalkyl group. The studied cyanines allow the detection of fibrillar aggregates in the wide range up to 0.8 to 300 μg/ml and permit monitoring the protein aggregation kinetics with high reproducibility. The modification of trimethine cyanine dyes by functional substituents in N,Nâ²-positions is suggested as a tool for the design of fluorescent molecules with the enhanced fluorescent sensitivity to the fibrillar aggregates of proteins.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Marina V. Kuperman, Svitlana V. Chernii, Mykhaylo Yu. Losytskyy, Dmytro V. Kryvorotenko, Nadiya O. Derevyanko, Yurii L. Slominskii, Vladyslava B. Kovalska, Sergiy M. Yarmoluk,