| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7559042 | Analytical Biochemistry | 2014 | 12 Pages |
Abstract
Protein modification has been found to affect the estimation of protein concentration in some of the traditional dye-based absorbance measurements. In this work, a distinct reduction in A595 was observed during the quantitation of a PEGylated exendin-4 analogue (Ex4C) by the Bradford method and the PEGylation process was found to interfere with the measurement. Lys12, Arg20, and Lys27 were further proved to be the major amino acids that functioned as dye-binding sites. The shielding effect produced by the large polymer was demonstrated to depend on the length of PEG that was used for modification.
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Physical Sciences and Engineering
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Analytical Chemistry
Authors
Xiaowei Qian, Hongxia Dong, Xiaojing Hu, Hong Tian, Linfeng Guo, Qingliang Shen, Xiangdong Gao, Wenbing Yao,