Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7559311 | Analytical Biochemistry | 2014 | 7 Pages |
Abstract
We describe a simple protocol for the preparation and orthogonal hydrophobic/hydrophilic LC-MS/MS analysis of mouse and human plasma samples, which enables the untargeted (“shotgun”) or targeted profiling of hydrophilic, amphipathic, and hydrophobic constituents of plasma metabolome. The protocol is rapid, efficient, and reliable, and offers several advantages compared to current procedures. When applied to a training set of human plasma samples, the protocol allowed for the rapid acquisition of full LogP metabolic profiles in plasma samples obtained from cognitively healthy human subjects and age-matched subjects with mild cognitive impairment or Alzheimer's disease (n = 15 each). Targeted analyses confirmed these findings, which are consistent with data previously published by other groups.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Andrea Armirotti, Abdul Basit, Natalia Realini, Carlo Caltagirone, Paola Bossù, Gianfranco Spalletta, Daniele Piomelli,