Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7559541 | Analytical Biochemistry | 2013 | 5 Pages |
Abstract
In this study, a highly sensitive capillary-based enzyme-linked immunosorbent assay (ELISA) has been developed for the analysis of picomolar levels of thrombin-cleaved osteopontin (trOPN), a potential biomarker for ischemic stroke, in human plasma. Using a square capillary coated with 8.5 μg/ml anti-human trOPN capture antibody for ELISA, the linear range obtained was 2 to 16 pM trOPN antigen. This concentration range was in the detection window of trOPN antigen in plasma samples. Compared with the conventional microplate-based ELISA, the current capillary technique significantly reduced the amounts of reagent from milliliter to microliter, reduced the analysis time from 8 to 3 h, and had a better sensitivity and detection limit performance from approximately 50 pM down to 2 pM of trOPN antigen. These results indicate that this capillary-based immunoassay is a potential tool for biomarker detection and may be useful in clinical trials and medical diagnostic applications.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Shun-ichi Funano, Terence G. Henares, Mie Kurata, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto,