| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7564533 | Chinese Journal of Analytical Chemistry | 2017 | 6 Pages |
Abstract
This study systematically evaluated the different results of trypsin digestion and Lys-C/trypsin sequential digestion used in proteomics sample paration. The results indicated that the numbers of identified peptides and proteins increased significantly through Lys-C/trypsin sequential digestion. Besides, the usage amount of enzymes in Lys-C/trypsin sequential digestion was optimized. The study provided references for conducting high efficient proteomics sample preparation.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Qian LI, Yu FENG, Min-Jia TAN, Lin-Hui ZHAI,