Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7583826 | Food Chemistry | 2019 | 6 Pages |
Abstract
An anaerobic incubation for the enzymatic production of gamma-aminobutyric acid (GABA) in rice bran with the addition of glutamate, hydrolyzed wheat protein (HWP), yeast extract (YE) and pyridoxal-5-phosphate (PLP) was investigated. Rice bran was moistened (30% moisture content) with an electrolyzed oxidizing water and anaerobically incubated under nitrogen at 40â¯Â°C for 8â¯h. The incubation activated the glutamate decarboxylase (GAD) in rice bran and increased the GABA content from 10.7 to 171.5â¯mg/100â¯g. The addition of glutamate and protein hydrolysates further amplified the GABA content in the treated rice bran: 974.9, 487.4, and 372.8â¯mg/100â¯g, with 2.25% glutamate, 6% HWP, and 8% YE, respectively. Furthermore, addition of PLP (1.48â¯mg/100â¯g) as a coenzyme for GAD, together with 2.25% glutamate addition, could raise the GABA accumulation in rice bran to 2242â¯mg/100â¯g. Other amino acids in rice bran were changed in their composition by the anaerobic treatment.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Su-Jin Oh, Hyun Soo Kim, Seung-Taik Lim, Chagam Koteswara Reddy,