Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7583884 | Food Chemistry | 2019 | 8 Pages |
Abstract
Perchlorate (ClO4â) and bromate (BrO3â) are mainly determined by ion chromatography methods. The present work aims to develop a modified QuEChERS combined with ultrahigh performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for simultaneous analysis of ClO4â and BrO3â in fruits and vegetables including cherry tomato, strawberry, tomato, potato, lettuce and pakchoi. Two target inorganic anions were separated on a Diamonsil C18(2) column by gradient elution, scanned in ESI negative mode and detected under multiple reactions monitoring (MRM). Limit of detection (LOD) and method limit of quantification (MLOQ), matrix effect, accuracy and precision of the developed method were investigated. Results were linear in their concentration ranges, with coefficients of determination (r2) bigger than 0.999. LODs for ClO4â and BrO3â were 0.1â¯Î¼g/L and 0.5â¯Î¼g/L, respectively. The validated method provided sufficient selectivity, sensitivity and accuracy to screen for ClO4â and BrO3â with ignorable matrix effects. Recoveries for different kinds of negative samples at 3 spiked levels were between 82.0% and 99.5%. Both intraday-precision and interday-precision were satisfactory, showing RSDr values in the range of 2.1-7.1% and RSDR values in the range of 7.2-10.8%. The proposed method was successfully applied to determine target inorganic anions in several fruits and vegetables. Perchlorate was detected in 12 vegetable samples with the concentrations ranged from 21â¯Î¼g/kg to 162â¯Î¼g/kg. Results indicated that there was wide ClO4â contamination in vegetables, confirming its urgency for monitoring harmful inorganic anions in our daily consumed vegetables.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Hao Dong, Kaijun Xiao, Yanping Xian, Yuluan Wu, Liang Zhu,