Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7583934 | Food Chemistry | 2019 | 6 Pages |
Abstract
The aim of this study was to synthesize stearidonic acid (SDA)-rich triacylglycerol (TAG) via a two-step lipase-catalyzed esterification under vacuum. SDA-rich fatty acid, which was prepared from echium oil via Candida rugosa lipase-catalyzed selective esterification, was used as the substrate. Two different immobilized lipases, Novozym 435 from Candida antarctica and Lipozyme TL IM from Thermomyces lanuginosus, were employed for the synthesis of SDA-rich TAG. In the first step, Novozym 435-catalyzed esterification of the SDA-rich fatty acid with glycerol was carried out for 2â¯h. In the second step, Lipozyme TL IM-catalyzed esterification of the reaction mixture from the first step was performed for an additional 10â¯h. The optimal reaction conditions for the second step were a temperature of 65â¯Â°C, an enzyme loading of 20%, and a vacuum of 0.7â¯kPa. Consequently, the maximum TAG conversion of ca. 86.4â¯wt% was obtained after 12â¯h via a two-step lipase-catalyzed esterification.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Nam Ho Kim, Heejin Kim, Nakyung Choi, Yangha Kim, Byung Hee Kim, In-Hwan Kim,