Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7584017 | Food Chemistry | 2019 | 33 Pages |
Abstract
In this study, a simultaneous analytical method of tocols, γ-oryzanols, phytosterols, squalene, cholecalciferol and phylloquinone were developed using HPLC-DAD-FLD. The developed method allowed the quantification of 18 compounds in 30â¯min. Method validation showed linearity of calibration curves (αâ¯=â¯0.05). RSD of intra-day, inter-day and inter-laboratory precision were less than 4.88%. The limit of detections (LODs) and limit of quantifications (LOQs) were low (0.009-2.166â¯Î¼gâ¯gâ1) with recoveries around 96.0-102.9%. Results derived from the established method demonstrated a wide variation of detected compounds in rice bran and vegetable oil samples (22.4-1774.6â¯Î¼gâ¯gâ1 tocols, ND-26484â¯Î¼gâ¯gâ1 γ-oryzanols, ND-12655â¯Î¼gâ¯gâ1 phytosterols, ND-3189â¯Î¼gâ¯gâ1 squalene, ND-105.3â¯Î¼gâ¯gâ1 cholecalciferol, and ND-54.4â¯Î¼gâ¯gâ1 phylloquinone). Thus, the developed HPLC-DAD-FLD method is a powerful analytical tool for the above mentioned compounds useful in food and pharmaceutical application.
Keywords
β-Tocopherol (PubChem CID: 6857447)δ-Tocopherol (PubChem CID: 92094)Stigmasterol (PubChem CID: 5280794)Campesterol (PubChem CID: 173183)Cholecalciferol (PubChem CID: 5280795)β-sitosterol (PubChem CID: 222284)squalene (PubChem CID: 638072)α-Tocopherol (PubChem CID: 14985)AntioxidantsRiceSimultaneous analysisOilLipidsHPLC
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Piramon Pokkanta, Phumon Sookwong, Manatchanok Tanang, Saranya Setchaiyan, Pittayaporn Boontakham, Sugunya Mahatheeranont,