Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7584210 | Food Chemistry | 2018 | 10 Pages |
Abstract
To date numerous metabolomic studies have been performed in order to characterize nutritional intervention studies. The aim of the current study was to present a comprehensive pipeline for characterizing the metabolic changes that occur in chickens tissues in response to naringin and hesperidin dietary supplementation. Forty-nine chickens were randomly divided into 3 groups: the first one fed with diet supplemented with naringin, the second with hesperidin whereas the control group was fed by commercial basal diet. After 30â¯days of administration chicken muscle samples were analyzed by UHPLC-HRMS whereas data were analyzed by the proposed pipeline. Three significant variables were detected to discriminate the control from the group after naringin administration and thirteen variables after hesperidin supplementation. Furthermore, a more detailed pipeline (encompassing multiple internal standards, internal validation of the clustering, extended statistical significance scores and multiple identification procedures) has been proposed aiming towards a more accurate untargeted analysis.
Related Topics
Physical Sciences and Engineering
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Analytical Chemistry
Authors
Eirini Baira, Ioanna Dagla, Eleni Siapi, Panagiotis Zoumpoulakis, Panagiotis Simitzis, Michael Goliomytis, Stelios G. Deligeorgis, Alexios-Leandros Skaltsounis, Evagelos Gikas,