Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7584371 | Food Chemistry | 2018 | 8 Pages |
Abstract
The effect of hydrogenation of phospholipids on the characteristics of freeze-dried liposomes was investigated using hydroxypropyl-Ã-cyclodextrin (HP-Ã-CD) as membrane protectant. The ethanol-injection method was applied to prepare liposomes using hydrogenated (Phospholopion-90H and 80H) and non-hydrogenated phospholipids (Lipoid-S100) in combination with cholesterol. Various liposomal formulations were tested: conventional liposomes (CL) and HP-Ã-CD-loaded liposomes (CDL). Liposome suspensions were concentrated by ultracentrifugation; the pellets were reconstituted in water or CD solution and the dispersions were characterized for their size, polydispersity index and zeta potential. Results demonstrated that HP-Ã-CD protected only the hydrogenated batches (CL and CDL) during freeze-drying. Moreover, the presence of HP-Ã-CD in the aqueous phase of CDL protected them during freeze-drying. Freeze-dried CL and CDL made of phospholipon-90H loading anethole were demonstrated to be physically stable upon reconstitution in HP-Ã-CD solutions, and are able to retain anethole after 6â¯months of storage at 4â¯Â°C thereby making them valuable for food applications.
Related Topics
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Authors
Riham Gharib, Hélène Greige-Gerges, Sophie Fourmentin, Catherine Charcosset,