Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7584375 | Food Chemistry | 2018 | 24 Pages |
Abstract
Cu could act as a modifier and influence lipid metabolism, but the potential mechanism was not explored. Juvenile yellow catfish were fed diet containing 0.71 (low Cu), 3.93 (intermediate Cu) and 88.81 (high Cu) mg Cu kgâ1, for 8â¯weeks to explore the modulation of intestinal lipid metabolism following dietary Cu addition. Using specific pathway inhibitors (Fatostatin for SREBP1, T0070907 for PPARG and Compound C for AMPK), primary enterocytes of yellow catfish were used to explore the molecular mechanisms of Cu reducing intestinal lipid deposition. Dietary Cu addition triggered Cu accumulation but suppressed lipid deposition in the fore- and mid-intestine. The reduced lipid deposition was attributable to the suppressed lipogenesis and lipid absorption, and accelerated lipid transport. The PPARG, SREBP1 and AMPK signaling pathways mediated the Cu-induced changes in lipogenesis, lipid uptake and lipid transport in the intestine of yellow catfish.
Keywords
qPCRSGRIBWPPARCD36ICP-AESFBWFASFATPG6PDReal-time fluorescence quantitative PCRICDHMTPLPLFABPSREBPACCAMPKAMP-activated protein kinaseH&Emalic enzymeapoapolipoproteinacetyl-CoA carboxylaseinductively coupled plasma atomic emission spectrometryfatty acid synthaseWeight gainfatty acid translocaseIsocitrate dehydrogenaseANOVAone-way analysis of variancecompound Ctriacylglycerolstandard error of meansLipoprotein lipaseLipid metabolismSEMCopperSignaling pathwaysFeed intakeSurvival ratespecific growth ratehematoxylin-eosinFatty acid-binding proteinmicrosomal triglyceride transfer proteinFatty acid transport proteinchylomicronsglucose 6-phosphate dehydrogenaseperoxisome proliferators-activated receptor
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Guang-Hui Chen, Zhi Luo, Christer Hogstrand, Kun Wu, Shi-Cheng Ling,