Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7584834 | Food Chemistry | 2018 | 8 Pages |
Abstract
An analytical method for the non-target screening of macrolides and metabolites in bass (Lateolabrax) was developed using an automated on-line extraction procedure followed by ultrahigh-performance liquid chromatography coupled to electrospray ionization quadrupole Orbitrap high-resolution mass spectrometry (UHPLC Q-Orbitrap). The estimated performance characteristics were satisfied, complying with the requirements of the guidelines specified in European Commission Decision 2002/657/EC. The decision limit ranged from 0.12â¯Î¼gâ¯kgâ1 to 3.61â¯Î¼gâ¯kgâ1, and detection capability ranged between 0.20â¯Î¼gâ¯kgâ1 and 6.02â¯Î¼gâ¯kgâ1. Precision in terms of relative standard deviation (RSD) was under 14% for all compounds, and the extraction recoveries ranged from 81% to 107%. Finally, the method was applied to ten different commercially important bass species and confirmed the presence of ten macrolides and metabolites. Five non-target compounds of robenidine, lincomycin hydrochloride, thiacloprid, fenbendazole, and thiabendazole were elucidated in the untargeted screening.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Wei Jia, Lin Shi, Xiaogang Chu, James Chang, Ying Chen, Feng Zhang,