Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7585196 | Food Chemistry | 2018 | 25 Pages |
Abstract
α-Glucosidase is an enzyme that plays a key role in raising blood sugar level and is considered a good target for developing drugs to treat type 2 diabetes. This study was performed to evaluate the inhibition of the catalytic reaction of α-glucosidase by minor phlorotannin derivatives (1-5) from Ecklonia cava. These derivatives demonstrated inhibitory activity, with IC50 values ranging from 2.3â¯Â±â¯0.1 to 59.8â¯Â±â¯0.8â¯Î¼M. Among the phlorotannins identified, compounds 2 and 3-5 were revealed to be non-competitive and competitive inhibitors, respectively. Furthermore, a fluorescence-quenching study of receptor-ligand binding was performed to calculate the kinetic parameters (Ksv, Kq, and K). These signal data indicated a 1:1 ratio of ligand-receptor binding. The binding conformations of the phlorotannin ligands were visually solved through molecular simulation. In conclusion, these minor phlorotannins may serve as α-glucosidase inhibitors targeted for the treatment of type 2 diabetes.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Sae Rom Park, Jang Hoon Kim, Hae Dong Jang, Seo Young Yang, Young Ho Kim,