Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7585286 | Food Chemistry | 2018 | 8 Pages |
Abstract
An accelerated solvent extraction (ASE) with in-cell clean-up method coupled to ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed to determine 21 antibiotics in sea cucumber. The analytes include 10 sulfonamides, 4 fluoroquinolones, 3 amphenicols, 2 beta-lactams, 1 lincosamide and trimethoprim. Optimal parameters of ASE method were obtained at 80â¯Â°C, 1 static cycle of 5â¯min with methanol/acetonitrile (1/1, v/v) using 2â¯g of C18 as adsorbent. Recoveries at 50.1-129.2% were achieved with RSD under 20%. Method detection limits ranged from 0.03 to 2.9â¯Î¼gâ¯kgâ1. Compared to the reported ultrasound-assisted extraction method, the proposed method offered comparable extraction efficiency for sulfonamides from sea cucumber, but higher for other categories of antibiotics. This validated method was then successfully applied to sea cucumber samples and 9 antibiotics were detected with the highest concentration up to 57.7â¯Î¼gâ¯kgâ1 for norfloxacin.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Minghua Zhu, Hongxia Zhao, Deming Xia, Juan Du, Huaijun Xie, Jingwen Chen,