Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7585428 | Food Chemistry | 2018 | 31 Pages |
Abstract
The present paper describes the validation of a spectrophotometry method involving molecular absorption in the visible ultraviolet-visible (UV-Vis) region for selenium (Se) determination in the bacterial biomass produced by lactic acid bacteria (LAB). The method was found to be suitable for the target application and presented a linearity range from 0.025 to 0.250â¯mg/L Se. The angular and linear coefficients of the linear equation were 1.0678 and 0.0197â¯mg/L Se, respectively, and the linear correlation coefficient (R2) was 0.9991. Analyte recovery exceeded 96% with a relative standard deviation (RSD) below 3%. The Se contents in LAB ranged from 0.01 to 20â¯mg/g. The Se contents in the bacterial biomass determined by UV-Vis were not significantly different (pâ¯>â¯0.05) those determined by graphite furnace atomic absorption spectrometry. Thus, Se can be quantified in LAB biomass using this relatively simpler technique.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Ana Paula Mörschbächer, Anja Dullius, Carlos Henrique Dullius, Cassiano Ricardo Bandt, Daniel Kuhn, Débora Tairini Brietzke, Fernando José Malmann Kuffel, Henrique Pretto Etgeton, Taciélen Altmayer, Tamara Engelmann Gonçalves,