| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7585573 | Food Chemistry | 2018 | 8 Pages |
Abstract
CdTe/CdS/ZnS quantum dots (QDs) were synthesized in aqueous phase and conjugated with a new anti-zearalenone (ZEN) monoclonal antibody. Using this novel fluorescent probe, a fluoroimmunoassay (FLISA) and a rapid immunochromatographic strip (ICTS) were developed for the detection of ZEN in maize. Our proposed FLISA allowed for ZEN determination in the range of 0.038-0.977â¯ng/mL with an IC50 of 0.162â¯ng/mL and a limit of detection (LOD) of 0.012â¯ng/mL occurring in maize. The rapid ICTS had a visual LOD of 1.0â¯ng/mL in buffer solution and 1.5â¯ng/mL in maize extract. These two QDs-based immunoassays were all successfully verified by commercial ELISA kits. The results confirmed that: firstly, the FLISA can be used as a robust method for the sensitive detection of ZEN; and secondly, the ICTS is ideally suited for rapidly screening large numbers of samples.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Fuyuan Zhang, Bing Liu, Wei Sheng, Yan Zhang, Qi Liu, Shijie Li, Shuo Wang,