Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7585710 | Food Chemistry | 2018 | 49 Pages |
Abstract
This article reviews recent developments in methods of sample preparation and analytical methodologies for the quantification of anthocyanins and their extraction from food sources. Various methods for sample extraction and purification are highlighted and evaluated. The use of UV-diode array, along with improved liquid chromatography (LC) and mass spectrometry (MS) and/or the combination of both methods have facilitated the identification of analytes. The use of one-dimensional and two-dimensional HPLC has significantly improved resolution with a shorter amount of time. Other LCâ¯Ãâ¯LC combinations to improve orthogonality are also discussed. The most efficient anthocyanin extraction method from food sources is pressurized liquid extraction. Moreover, electrospray ionization (ESI) and MS2/time-of-flight are currently the most popular instruments used for identification of anthocyanins; being positive mode of ESI the most widely used procedure for anthocyanin identification. Several databases for mass spectrometry polyphenol identification have been described for reference.
Keywords
UPLCACNPHEUHPLCTFASPEIMSDADAOACPLEL-phenylalanineGMDGlcTrpTOFqTOFEPLESIHSCCCRP-LCXylQqQIM-MSArabinosenuclear magnetic resonancel-Tyrosinel-Tryptophandiode array detectorAnthocyaninAnthocyaninsSolid phase extractionPressurized Liquid ExtractionTrifluoroacetic acidassociation of official analytical chemistsNMRIon trapTyrmatrix-assisted laser desorption/ionizationrhamnoseResponse surface methodologyRSMTime-of-Flight quadrupole time-of-flightIon mobility mass spectrometryMass spectrometryIon mobility spectrometryArabultra-violetFlavonoidsMALDIHILICTriple quadrupoleHigh-speed counter-current chromatographyhydrophilic interaction chromatographyliquid chromatographyhigh-pressure liquid chromatographyReversed-phase liquid chromatographyUltra-high-pressure liquid chromatographyHPLCGas chromatographyGalGalactoseXyloseGlucoseelectrospray ionization
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Paulina Ongkowijoyo, Diego A. Luna-Vital, Elvira Gonzalez de Mejia,