Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7585907 | Food Chemistry | 2018 | 25 Pages |
Abstract
In the present study an optimization of trienzyme treatment combining α-amylase, protease and γ-carboxy peptidase allowing complete sample preparation within a working day for the analysis of vitamin B9 (folate) in infant formula and adult/pediatric nutritional products is presented. The optimized sample preparation was applied to a set of samples representing most of the products in the marketplace. Results on Standard Reference Material 1849a were well in agreement with certified values. The main contributor to total folate was folic acid, 5-methyl-tetrahydrofolate was the only minor contributor in milk-based products. Soy-based formulas contained polyglutamates of 5-formyl-tetrahydrofolate. The relative contribution of polyglutamates to the total folate content remained low in the types of product included in this study. The results suggest that a simple di-enzyme treatment could be enough for these products, nevertheless, this should be carefully evaluated prior to making a decision on the use of tri- or di-enzyme treatment.
Keywords
SAXSRMDRISPEUHPLC5MTHFRTFUPLC5-Methyltetrahydrofolate5-Formyltetrahydrofolate5,10-methenyltetrahydrofolateDfEMS/MSSolid phase extractionFolic acidstrong anion exchangeInfant formulaInfant milkMass spectrometryTandem mass spectrometryFolateClinical nutritionstandard reference materialDietary reference intakeUltra High Performance Liquid Chromatography
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Esther Campos-Giménez, Sylvie Bénet, Yannick Oguey, Frédéric Martin, Karine Redeuil,