Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7585927 | Food Chemistry | 2018 | 8 Pages |
Abstract
The aim of this work was the development of a simple, novel and accurate method for the determination of adenosine triphosphate (ATP) and its first five catabolites: adenosine diphosphate (ADP), adenosine monophosphate (AMP), inosine monophosphate (IMP), inosine (Ino) and hypoxanthine (Hx), in fish tissue, based on hydrophilic interaction liquid chromatography (HILIC). For this purpose, a stationary phase for polar and hydrophilic compounds (ZIC-pHILIC) was used. The effect of different chromatographic parameters and the molecular mechanism based on the van't Hoff plot were examined. The t-test and Dixon's Q-test were applied in order to examine statistical differences and outlier values. The recovery of the method ranged between 82.7% and 127% and the %RSD values were lower than 10% for all analytes determined. The method was applied in frozen sea bream samples stored at 0-4â¯Â°C. The Ki-, G-, H- and F values were calculated for the estimation of the level of fish freshness.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Maria Logotheti, Konstantina Theochari, Marios Kostakis, Ioannis N. Pasias, Nikolaos S. Thomaidis,