Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7587300 | Food Chemistry | 2017 | 6 Pages |
Abstract
The Next Generation Sequencing methodologies are considered the next step within DNA-based methods and their applicability in different fields is being evaluated. Here, we tested the usefulness of the Ion Torrent Personal Genome Machine (PGM) in food traceability analyzing candies as a model of high processed foods, and compared the results with those obtained by PCR-cloning-sequencing (PCR-CS). The majority of samples exhibited consistency between methodologies, yielding more information and species per product from the PGM platform than PCR-CS. Significantly higher AT-content in sequences of the same species was also obtained from PGM. This together with some taxonomical discrepancies between methodologies suggest that the PGM platform is still pre-mature for its use in food traceability of complex highly processed products. It could be a good option for analysis of less complex food, saving time and cost per sample.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Marta Muñoz-Colmenero, Jose Luis MartÃnez, AgustÃn Roca, Eva Garcia-Vazquez,